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Welcome to the solid phase immunoassay website!


The introduction of solid phase immunoassays for the detection and characterization of HLA antibodies in histocompatibility laboratories has dramatically changed the way laboratories approach the testing and immunologic assessment of transplant patients.  These assays have allowed histocompatibility laboratories to provide the clinician with valuable information for the management of patients undergoing a wide range of treatment modalities customized to the transplant centers’ protocols.  To date there is no single site (resource) where laboratorians can access the most current findings which can affect the use of these assays in the lab and obtain help in trouble shooting, test interpretation and a general exchange of information regarding these assays.

We have created a website to address these issues.  The intent of the website is to collect a variety of data on solid phase immunoassays that will cover both the technical elements of the assays and the application of the assays to clinical parameters

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  • O.O.Adebiyi, J.Gralla, P.Klem, B.Freed, S.Davis, A.C.Wiseman and J.E.Cooper

    Introduction
    The widespread utilization of single-antigen bead (SAB) assays prior to kidney transplantation has improved the detection of donor-specific antibody (DSA) in potential transplant recipients. Many studies have documented inferior renal graft outcomes in the presence of pretransplant DSA (1–15). However, the clinical importance of such DSA in the setting of a negative flow cytometry crossmatch (FCXM) at transplant remains controversial and the need for desensitization uncertain. There are limited published data regarding the impact of specific DSA characteristics such as class, strength or whether DSA persisted posttransplant on renal allograft outcomes when flow cytometry is negative. Additionally, the clinical significance…

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  • Dolores Redondo-Pachón, Julio Pascual, María J.Pérez-Sáez, Carmen García, Juan José Hernández, Javier Gimeno, Marisa Mir, Marta Crespoa

    The influence of antibodies against HLA-DP antigens detected with solid-phase assays on graft survival after kidney transplantation (KT) is uncertain.We evaluated with Luminex®the prevalence of pre- and posttransplant DP antibodies in 440 KT patients and their impact on graft survival. For 291 patients with available pretransplant samples, DP antibodies were present in 39.7% KT with pretransplant HLA antibodies and 47.7% with DSA. Graft survival of KT with pretransplant class-II DSA was worse than with non-DSA (p= 0.01). DP antibodies did not influence graft survival. Of 346 patients monitored post-KT, 17.1% had HLA class-II antibodies, 56% with DP antibodies. Class-II DSA was detected in 39%, 60.9% of them had DP antibodies. Graft survival was…

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  • Craig J.Taylor, Vasilis Kosmoliaptsis, Jessie Martin, Graham Knighton, Dermot Mallon, J. Andrew Bradley, and Sarah Peacock

    Background. Solid-phase assays to distinguish complement binding from noncomplement binding HLA-specific antibodies have been introduced, but technical limitations may compromise their interpretation. We have examined the extent to which C1q-binding to HLA-class I single-antigen beads (SAB) is influenced by denatured HLA on SAB, antibody titre, and complement interference that causes a misleading low assessment of HLA-specific antibody levels. Methods. Sera from 25 highly sensitized patients were tested using Luminex IgG-SAB and C1q-SAB assays. Sera were tested undiluted, at 1:20 dilution to detect highlevel IgG, and after ethylene diamine tetraacetic acid treatment to obviate complement interference. Conformational HLA and denatured HLA…

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  • Glen P.Westall, and Greg I.Snell

    The lung transplant community continues to struggle with the diagnosis and management of antibody-mediated rejection. The four diagnostic tenets of donor-specific antibodies, C4d staining, histopathologic changes, and allograft dysfunction, which were largely derived from the early Banff meetings on renal transplantation, have somewhat arbitrarily been applied to lung transplantation. With the passage of time, it is increasingly apparent that merits of these diagnostic pillars are less robust in lung transplantation. In this article, we summarize some of the controversies and challenges surrounding the diagnosis of antibody-mediated rejection in lung transplantation.

    Keywords: Lung transplantation, Antibody-mediated rejection, Humoral rejection, Chronic lung allograft syndrome.…

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Jessica Loescher is now a member of Solid Phase Immunoassays
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O.O.Adebiyi, J.Gralla, P.Klem, B.Freed, S.Davis, A.C.Wiseman and J.E.Cooper
Introduction
The widespread utilization of single-antigen bead (SAB) assays prior to kidney transplantation has improved the detection of donor-specific antibody (DSA) in po…
Immunoassays’s blog post was featured
Dolores Redondo-Pachón, Julio Pascual, María J.Pérez-Sáez, Carmen García, Juan José Hernández, Javier Gimeno, Marisa Mir, Marta Crespoa
The influence of antibodies against HLA-DP antigens detected with solid-phase assays on graft survival after kidn…
Immunoassays’s blog post was featured
Craig J.Taylor, Vasilis Kosmoliaptsis, Jessie Martin, Graham Knighton, Dermot Mallon, J. Andrew Bradley, and Sarah Peacock
Background. Solid-phase assays to distinguish complement binding from noncomplement binding HLA-specific antibodies have been…
Fadia H Jenkins is now a member of Solid Phase Immunoassays
Ron Kerman , PhD is now a member of Solid Phase Immunoassays
Joseph Kelly is now a member of Solid Phase Immunoassays
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