Flow Cytometry Single Antigens

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  • The FlowPRA Single Antigen Class I and II Antibody Detection Test and the FlowPRA Specific Class I and II Antibody Detection Test both include four different vials with four different groups used in identifying either Class I or II IgG antibodies.  The FlowPRA Single Antigen Class I as well as the Class II Antibody Detection assay are comprised of only 32 single antigens.   In order to run a batch of ten patient samples with a positive and negative control, the run size for the Class I assay alone is 48 tubes.  Testing both Class I and Class II on ten patient samples would result in a run size of 96 tubes.  Also, each group requires 20 µL of serum to be added, therefore 160 µL of serum is required to run both Class I and II on either the single antigen or specificity assay.  Since these assays are labor intensive and utilize more serum than other techniques, this question arises: How are these assays being utilized in each laboratory?  Are they being used as a primary identification tool or a supplement to aid in complicated cases?  If these assays are being used as primary identification tool, is it difficult to manage turn-around times?  Since these assays are less expensive than the Luminex based tests, are they being used to monitor known antibody on a monthly or quarterly basis in a alternating rotation with a Luminex based assay?  Since all laboratories struggle with the balance of budget and providing the best quality results, a discussion on this subject could prove helpful and enlightening. 

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