"It makes perfect sense. How many washes do you do after the first incubation step when using the manifold? Could you off-set poor washing that you get with low vaccuum pressure simply by increasing the number of washes? In my experience, increasing…"
Thanks for your comments. Yes, we just started validating the rapid protocol using filter plate washes and the preliminary results look good. In addition, two labs that participated in the multicenter ROB protocol evaluation study (late…"