Solid Phase Immunoassays

single antigen panel (1)

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  HLA Antibody Detection and Characterization by Solid Phase Immunoassays: Methods and Pitfalls

  Posted by Immunoassays on June 28, 2013 at 4:39pm

  Andrea A. Zachary , Renato M. Vega , Donna P. Lucas , and Mary S. Leffell

  Abstract
  Solid phase immunoassays for the detection and characterization of HLA-speci fi c antibodies provide greatly increased sensitivity, speci fi city, and time and reagent ef fi ciency, compared to the traditionally used cell-based methods. Testing is performed using commercially available test kits. The assays are of two general types: enzyme-linked immunosorbent assays and multianalyte bead. The types vary in both sensitivity
  and equipment requirements.
  While these assays afford great improvement over the cell-based assays, they can be confounded by interference from substances within the serum that result in high background reactivity. The high sensitivity of the assays also makes them more susceptible to environmental factors and operator variability. The user must be…

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  • Tags: enzyme-linked immunosorbent assays, hla antibody, hla phenotype panel, luminex ®, multianalyte bead assays, pooled antigen panel, single antigen panel, solid phase immunoassays

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